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Image Search Results
Journal: STAR Protocols
Article Title: Generation of a CHIP isogenic human iPSC-derived cortical neuron model for functional proteomics
doi: 10.1016/j.xpro.2022.101247
Figure Lengend Snippet:
Article Snippet:
Techniques: Recombinant, Sequencing, Modification, Ligation, Reverse Transcription, Plasmid Preparation, Purification, Cloning, CRISPR, Control, Software, Data-independent acquisition, Membrane, Mass Spectrometry
Journal: bioRxiv
Article Title: Genetic approaches in mice demonstrate that neuro-mesodermal progenitors express T/Brachyury but not Sox2
doi: 10.1101/503854
Figure Lengend Snippet: ( a ) Sox2 protein and mRNA levels are down-regulated in n = 5/5 (immunostaining) and n = 4/4 (WISH) T CreERT2/+ ; Sox2 f embryos. Sox2 staining: left side taken with the same exposure time as for wild-type (WT); right side taken with longer exposure time. Sox1 is not expressed at the caudal end of either WT nor in T CreERT2/+ ; Sox2 fl/fl embryos. Insets in a4 and a10 show cranial sections from the same embryo, stained and processed in parallel, as positive control for Sox1 antibody. Sox3 appears up-regulated in n = 5/5 T CreERT2/+ ; Sox2 fl/fl embryos compared to WT (n-values for WISH, IHC: WT, n = 3, 5 embryos; T CreERT2/+ ; Sox2 fl/fl , n = 4, 5 embryos). Dotted circles in a3 – 4 and a9 – 10 indicate the CNH. ( b ) Following multiple tamoxifen injections, n = 4/4 T CreERT2 +; Sox2 flfl embryos show normal neural tube morphology with normal dorso-ventral expression domains of Pax3, Pax6 and Nkx6.1 (n-values for WISH, IHC: WT, n = 3, 3 embryos; T CreERT2/+ ; Sox2 fl/fl , n = 3, 4 embryos). Dashed lines in a3 – 6, a9 – 12, b2 – 3, b5 – 6 outline the neuroepithelium. Scale bars: 1 mm for whole embryo ( b1 ), 500 μm for whole embryo and PNP ( a1 – 2 ); 100 μm for sections ( a3 – 4, b2 – 3 ).
Article Snippet: Primary antibodies: rabbit monoclonal anti-Sox2 (ab92494, Abcam, 1:500), chicken polyclonal anti-GFP (ab13970, Abcam, 1:500), mouse monoclonal anti-Sox10 (sc-365692, Santa Cruz, 1:500),
Techniques: Immunostaining, Staining, Positive Control, Expressing